Jun 2, 2010

Practice exam for Oral Histology

The images wouldn't copy onto blog - they are all on My Uni.


SECTION A

MULTIPLE CHOICE QUESTIONS – circle the correct answer in this test booklet.

1. Enamel is formed by which type of cell?

A) Odontoblasts
B) Enameloblasts
C) Ameloblasts
D) Fibroblasts

2. Enamel is usually % mineralised by weight:

A) 50
B) 65
C) 70
D) 96

3. Which of the following dental tissues are derived from the same embryonic source?

A) Enamel and dentine
B) Enamel and pulp
C) Enamel, dentine and pulp
D) Dentine and pulp

4. The peritubular dentine found surrounding each tubule is:

A) not true dentine
B) less calcified than intertubular dentine
C) more calcified than intertubular dentine
D) not different from intertubular dentine

5. In a healthy, mature tooth where is the location of the cell body of an odontoblast?

A) at the dentino-enamel junction
B) in the outer wall of the pulp
C) in the centre of the dentinal tubules
D) within the pulpal core

6. Secondary dentine forms:

A) before the completion of the apical foramen
B) in response to trauma
C) at the dentino-enamel junction
D) on the outside wall of the pulp

7. Enamel rods are longest:
(i) cusps tips
(ii) at the CEJ
(iii) incisal edges
(iv) at the bottom of fissures

A) i and ii
B) i and iv
C) ii and iii
D) i and iii


8. Incremental lines within the enamel that appear to transverse the rods are known as:

A) Lines of Retzius
B) Imbrication lines of Von Ebner
C) Enamel spindles
D) Perikymata


9. Which of the following properties is NOT characteristic of enamel?

A) Dynamic tissue
B) Highly mineralised
C) Nonvital
D) Highly vascular


10. Partially calcified enamel faults, which often extend from the DEJ to the outer surface, are known as:

A) Enamel tufts
B) Gnarled enamel
C) Enamel spindles
D) Enamel lamellae

PART B Short Answer Questions

1. Describe how fluoride interacts with enamel during amelogenesis and post-eruptively (5 marks)

2. You are placing a fissure sealant in a molar tooth. A step in the process is applying an acid etching solution to the enamel area to be sealed. Describe how the histology of enamel tissues enables the sealant to adhere to the tooth using the acid etch technique
(8 marks)



3. Describe the age changes that occur in the dental pulp? How do these changes affect the response of the dental pulp to an injury such as dental caries? (6 marks)

4. You are examining a patient and note that due to using a hard toothbrush and a vigorous action, they have worn away some of the enamel on the buccal surfaces of their molars.
4a What symptoms may these patients be experiencing and explain why?
(3 marks

4b What would this patient be at higher risk for on these affected surfaces? Give reasons for your answer.
(2 marks)
5. Ivor Payne has come into the surgery with a toothache in his upper left quadrant. Clinically only a small break in the enamel through an occlusal pit of 26 can be detected.

A bitewing radiograph of the area shows occlusal caries well established into dentine of the 26
5a Discuss the histological properties of enamel and dentine that explain why the caries appears radiographically.
(6 marks)
5b Why would Ivor be experiencing pain from the tooth?
(4 marks)
5c What mechanisms can the pulp initiate to protect itself from the bacterial invasion?
(3 marks)

6. The following diagram shows the developing dentine and enamel tissues during the apposition stage of tooth development.
Developing enamel
Diagram from Bath-Balogh M and Fehrenbach MJ (2006) Illustrated Dental Embryology, Histology and Anatomy, 2nd edition, Elsevier Saunders: St Louis
Developing dentine


6a Label the diagram where indicated. Then in the space below, discuss the purpose of each labelled feature (9 marks)

6b Describe what occurs to the ameloblasts and odontoblasts after formation of their relevant dental tissues, and what implications this has for the tooth.
(4 marks)
7. The junctional epithelium (JE) is considered to be the first line of defence that protects the underlying connective tissue from onslaught from bacterial toxins. How does the JE performs this function from a histological perspective.
(5 marks)

8. During a clinic session a patient presents with clinically healthy gingival tissues. (6 marks)

a. Describe how you would record the appearance of healthy gingival tissues on your examination sheet?
The presence of stippling is one indicator of gingival health. Explain what causes stippling on a histological level.
c. You notice that in one area, around the 34, there is an isolated area of 4-5 mm of recession. Outline any possible cause/s of this isolated case.


9. Explain how the periodontal ligament performs the following functions:
(7 marks)
Formative/regenerative
Nutritive
Sensory
Adaptive
Supportive

10. What radiographic indications could give you evidence of a healthy attachment apparatus? (4 marks)






11. Draw a series of clearly labelled diagrams to represent a tooth and its supporting structures. The following must be included: (pencil can be used to answer this question) (18 marks)

Enamel Alveolar crest
Dentine CEJ
Cementum Apex
Junctional epithelium Lamina dura
Sulcular epithelium Free gingiva
Gingival margin Attached gingiva
Mucogingival junction Alveolar mucosa
Free gingival groove Gingival col area

44 comments:

  1. 1. (c) Enamel is formed by Ameloblasts
    2. (D) Enamel is 96% mineralised
    3. (D) Dentine & Pulp are both derived from Mesenchyme?!
    4. (C) More calcified than intertubular dentine?

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  2. 5. (B) in the outer wall of pulp
    6. (C) on the outside wall of pulp????

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  3. Can anyone support Hannah here.... Lets get a bit of a tally board of correct answers happening so they we can all help each other through this.
    Does everyone agree with Hannah:
    1c, 2d., 3d., 4c., 5b., and 6c?

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  4. OK here goes!!! thought i would do the multiple choice ones 1st...then we can move on to the short answers!!

    1c,2d.,3a.,4c.,5b.,6c.,7d.,8a.,9d.,10d.

    we agreed on most Hannah...!! YAY...lets clarify question 3......mesenchyme cells sound right... i havent looked in my textbook for a day or so.....can anyone else clarify??? =)

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  5. 3. describe the age changes that occur in the dental pulp? how do these changes affect the response of the dental pulp to an injury such as caries?

    i understand the first part, but im confused with the second bit. Is this referring to how in the elderly, they have a less vascular pulp, so they are not able to remove disease as readily or is it talking about the tertiary dentine. The elderly don’t have as many cells, so they might not be able to form tertiary dentine?

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  6. Hi Jess
    think about the different types of cells that 'live' in the pulp and what happens to them as the pulp ages. Also consider how the continuous formation of secondary dentine 'shrinks'the pulp. This should help answer the question
    Cathy

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  7. So with question 3. would this then be talking about -
    • the pulp decreases in intercellular substance, water and cells and it fills with more collagen fibres
    • This decreases the number of cells (mesenchymal, white blood cells(Small amount), red blood cells (because of the high vascular supply)
    • Also the pulp cavity decreases in size as secondary dentine that is formed throughout life continually makes the pulp cavity smaller which makes it further away from the enamel and dentine which means there wont be a stimulus as quick, also due to decreasing size there will be less nerves?? And less stimulus due to that???
    Is that sort of along the lines of things???

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  8. 7. (d) i and iii ?
    8. (d) Perikymata ?
    9. (d) highly vascular
    10. (a) enamel tufts ?

    I got a bit confused with the ones with ?!!!!!!

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  9. Re the pulp question - think about the physiological impact on the pulp when the types cells (see text for details of which types of cell) which are abundant in a young pulp gradually decrease in number. You are on the right track re the shrinking nature of the pulp making it further from the external tooth surface.

    As for the MCQs - q 8 and 9 are correct. Can anyone help with q 7 and 10?

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  10. im still confused :S all I can think about is caries can progress through dentine quicker than enamel because it is less mineralized. But slightly off topic, with dentinogenesis, how much do we need to know about maturation of dentine. I looked in the text book and it was talking about globules and calcospherules etc and I have no idea what that means. I also looked in the lecture slides and saw there wasn’t a lot of detail regarding the maturation of dentine, but focused more on the maturation of enamel. Cheers.

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  11. How about because of the fibroblasts in the pulp - therefore it becomes more fibrouse and less vascular and less nerves?

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  12. Also with the MCQ - 10. (c) instead

    But I still don't know 7!!!!!!

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  13. hey guys
    multiple choice questions, i got??
    1. (C) Ameloblasts
    2. (D) 96%
    3. (D) Dentine and Pulp - Mesenchyme (Mesoderm)
    4. (C) more calcified
    5. (D) within the pulpal core
    6. (D) on the outside wall of pulp
    7. (D) i and iii
    8. (A) Lines of Retzius (occurs WITHIN the enamel, whereas Perikymata appears ON the surface)?????
    9. (D) Highly Vascular
    10. (D) Enamel Lamellae - hypomineralized that extends from DEJ to the surface of enamel. Enamel Tufts similar however not as extensive.

    HMMMMM when will we get the model answers so we can check??

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  14. the 'model' answers are all in your handouts and the Bath Balogh text. You will learn and retain the info so much better if you researc them, rather than me giving them to you - this leads to passive learning.

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  15. Reviewed answers for MCQs!!
    1. (c) Ameloblasts
    2. (d) 96%
    3. (d) Dentine and Pulp
    4. (c) More calcified than intertubular dentine
    5. (b) in the outer wall of the pulp
    6. (d) on the outside wall of the pulp
    7. d) cusp tips and incisal edges
    8. (d) perikymata
    9. (d) highly vascular
    10. (d) enamel lamellae
    !!!!!!!!

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  16. related to question 3:

    i also mentioned that because there would be less nerve cells, in older pulp, caries would progress more rapidly in teeth with older pulp.

    is this correct?

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  17. question 1 short answers -

    amelogenesis
    when fluoride is consumed, it is incoporated in the tooth structure forming fluoroapatite crystals. this is a systemic way. hence it requires a lower pH level to initiate the demin process. ( it feels like my explanation is incomplete please help)

    post-eruptively
    when demin process occurs, flouride will need to be applied topically. the accumulation of plaque produces bacterial acids which demins/breaks down the enamel and entine matrix. the bacterial acid that runs down the dentinal/enamel tubules leaches the minerals out. hence when flouride is applied topically, flouride ion run down the tubules along with acids which will eventually form flouroapatite crystals. this is called teh remin process.

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  18. With the mcq, I thought the answer to question 8 was a, lines of retzius and not d, perikymata. It says in the textbook ‘the lines of retzius appear as incremental lines… and seem to transverse the enamel rods.’ Page 186.

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  19. also, during amelogenesis, when the early enamel matrix is partially mineralised because ameloblasts pump calcium hydroxyapatite into it,does that mean if fluoride is consumed, ameloblasts pump fluoroapatite? and then during maturation, they pump more fluroapatite into the partially mineralised enamel matrix?

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  20. Jess i got the same as you for MCQ 3....Perikymata are the grooves evident on some teeth associated with the lines of Retzius..but appear on the outer enamel surface as opposed to within the enamel...so im assuming the correct answer is A??

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  21. * MCQ 8 not 3 for last comment*

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  22. Makes sense hey... but Cathy said Perikymata was correct?????????

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  23. helpful link guys..
    http://www.dental.pitt.edu/informatics/periohistology/en/gutoc.htm

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  24. Question 2. When the etch is placed on a enamel it removes the organic portion of the enamel between the enamel rods. This then creates a more porous surface and a larger surface area for the resin to flow into and lock on.

    Is that about right?

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  25. 3. Describe the age changes that occur in the dental pulp? How do these changes affect the response of the dental pulp to an injury such as dental caries?

    • Decrease in pulp volume
    • Reduction in the vascular supply
    • Reduction in cell density (undifferentiated mesenchymal cells, macrophages, lymphocytes, dendritic cells)
    • Increase in fibrous bundles (due to fibroblasts)
    • Decrease in innervation

    • This means the pulp is less inclined to send stimuli to the odontoblasts to make more tertiary dentine as a reaction to dental caries.
    • With age some of the dentinal tubules will have closed over with sclerotic dentine causing the stimuli from caries/cold/hot to become less to the pulp, therefore less pain is felt.

    ... WHAT ELSE?!

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  26. 2. You are placing a fissure sealant in a molar tooth. A step in the process is applying an acid etching solution to the enamel area to be sealed. Describe how the histology of enamel tissues enables the sealant to adhere to the tooth using the acid etch technique

    • Enamel is made up of many different enamel rods (prisms) surrounded by interrod space.
    • An exception of this is - the first layer placed (at DEJ), bottom of pits and fissures and the last layer placed. These areas have aprismatic enamel which is "roddless" enamel.
    • Aprismatic enamel occurs because when the ameloblasts are laying down enamel they dont have any abutting prisms in these areas for the rods to form.

    • Therefore the etch is placed onto the tooth to either remove a smear layer (caused by dental biofilm/cutting instruments) or to remove the aprismatic layer of enamel.
    • It then increases the porosity of enamel by selectively removing part of the organic matrix around the rods to increase surface area for better retention of restorative materials.

    ... ANY THING ELSE?

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  27. 1. Describe how fluoride interacts with enamel during amelogenesis and post-eruptively

    Pre-eruptively =
    • Through systematic factors - fluoride is taken up by the blood. The blood that is present during formation of the tooth will be incorporate nutrients/fluoride into the tooth structure.

    Post-eruptively =
    • Through the demin/remin cycle.
    • Through topical factors - toothpaste, saliva, gels

    DO WE NEED TO SAY WHAT DEMIN/REMIN CYCLE IS?????

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  28. 4. You are examining a patient and note that due to using a hard toothbrush and a vigorous action, they have worn away some of the enamel on the buccal surfaces of their molars.
    4a What symptoms may these patients be experiencing and explain why?

    • Sensitivity/Pain
    - Because the dentinal tubules are exposed.
    - Due to hydrodynamic theory, movement in the dentinal fluid (found inside tubules) can send a stimulus down towards the pulp which has contains nerves which will then be send stimulus to the brain, making pain.

    4b What would this patient be at higher risk for on these affected surfaces? Give reasons for your answer.

    • Dental caries
    - dentine is not as mineralised (70%) and an increase in organic material (30%) compared to enamel (96%) therefore caries can start/spread easier.

    • Gingival recession
    - ??????

    HELP PLEASE :D

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  29. Gingival recession- the patient would be more risk of tooth loss as loss of gingival tissue would result in decrease in alveolar crest height, periodontal disease and mobility, as well as root caries..???

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  30. True Rach! But would tooth brush abrasion cause recession?? If so how?

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  31. Hi from the US of A (Salt Lake City to be exact)
    have just checked out the Blog and it seems to be bubbling away very nicely.
    A couple of comments:
    Dentinogenesis issue raised by Jess a few comments ago - no need to get down to the 'globule' level, the details on dentinogenesis re the formation of odontoblasts and how the processes and tubules are formed is what you need to understand.
    Lines running within enamel at right angles to rods are the Lines of Retzius - the most superficial ones on the surface are known as perikymata.
    Pre- and post-eruptive action of Fl on enamel - not only it's source but the actual process of what happens to the formation of the enamel (or the remin if post-eruptive) if fluoride is present. How is the tissue different compared to if the fluoride wasn't there?

    Toothbrushes (especially hard ones combined with a vigorous style) can certainly cause recession.
    All from me - back to the pool and spa!
    C

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  32. Thanks Cathy! I hope the US of A is great :) Enjoy!

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  33. 7. The junctional epithelium (JE) is considered to be the first line of defence that protects the underlying connective tissue from onslaught from bacterial toxins. How does the JE performs this function from a histological perspective.

    -cells are loosely packed together, with fewer desmosomes junctions between the cells; the intercellular space is large
    -these features make the JE permeable
    -permeability allows for the movement of WBC into the JE and the underlying CT tissue
    -WBC help protect the tissues from microorganisms such as bacterial toxins

    am i missing anything?

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  34. For question10, I don't really understand what it is asking by the "attachment apparatus". Is that meant to be things like the Lamina Dura, cementum and PDL??

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  35. Ok Linda ..spot on with the JE question. Just add that it acts as a seal to protect the underlying tissues. The attachment apparatus is exactly the lamina dura, cementum and PDL... so well done!
    Sophie

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  36. in regards to the JE:
    permeable to tissue fluid.."Gingival Crevicular Fluid".. as the JE is non-keratinised, this poses as an exceptional challenge to the JE to protect the periodontium, and consequently the whole body against the sereral hundred bacterial species in the oral environment, the fluid is a key element to maintaining antimicrobial defense of the periodontium. Crevicular fluid contains serum, luekocytes, bacteria, ET cells, CT cells and bone cells...an increase in fluid would indicate increased bacterial numbers in the periodontium...this would lead to inflammation of the gingival tissue resulting in redness, loss of stippling, bleeding on probing.

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  37. Sounds good Rach - but doesn't the crevicular fluid come from the sulcus epithelium not junctional?

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  38. yes,but histologically the function of this fluid is to protect the JE
    here is an article i found on Pubmed:
    Gingival crevice fluid an introduction (p 9-11) Periodontol 2000. 2003;31.pdf
    this explains it pretty well and good diagram-
    if it doesnt work i can email it to u xxx

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  39. RANDOM QUESTION

    -what happens histologically when there is loss of stipplings?

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  40. Shubh, i think that has something to do with inflammation, which causes the rete ridges to expand and lose its structure, therefore loss of stipplings??

    i'm not 100% sure though...

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  41. yeah ...i had some idea along those lines....but i need specific info so i can clearly understand it! but now that u have confirmed that we r on the right track...hopefully some1 else can help us!

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  42. Yes Linda your right!
    Because of the inflammatory cells found in the CT when inflammation occurs fluid diffuses into the epithelium tissue above which then expands the basal lamina which causes the rete ridges to flatten out....

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  43. omg! thanks hannah....great explanation!

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  44. Well done guys.. also because inflammation produces enzymes that breakdown collagen, you lose the wavy interface between the ET and CT.

    The crevicular fluid originates from the underlying connective tissue and diffuses across the basement membrane into junctional epithelium and out into the sulcus. The fact that the junctional epithelial cells are attached by desmosomes, create larger intercellular spaces for this crevicular fluid to diffuse into the sulcus...

    Sophie

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